Faculty members located in academic
departments/centers can be formally associated with the IGBB in two
different ways:
1. IGBB Fellows
Through its Fellows Program, the
IGBB rewards those faculty who have worked within the IGBB to build a
strong, vibrant molecular biosciences/computational biology research
community at MS State. IGBB Fellows receive not only formal recognition
for their accomplishments, but they are given a voice in many aspects of
IGBB operation including, but not limited to, input on purchases, new hires,
and overall research direction. The IGBB Fellows literally serve as
the Director's Advisory Committee. To be an IGBB Fellow, a faculty member from an academic department
must (1) regularly submit proposals through the IGBB, (2) have had at least one funded external grant (with overhead)
administered through the IGBB, (3) have a record of high-impact scientific
productivity, and (4) have a national/international reputation in his/her
field. IGBB Fellows have full "internal user" access to the HPC² supercomputers and receive HPC²
computer, technical, and administrative support.
2. IGBB Faculty Affiliates
An IGBB Faculty Affiliate is any faculty member in an MS State academic department who (1) conducts research
in the molecular biosciences and/or computational biology and (2) collaborates in some manner with IGBB faculty/staff. Like IGBB Fellows, IGBB Faculty Affiliates are encouraged to submit proposals through the IGBB, but
are not obligated to do so. IGBB Faculty Affiliates generally do not
have HPC² supercomputer access unless they are working on an IGBB
research project. Faculty Affiliates can request access to the HPC²
supercomputers if they are interested in generating data/results for an eventual proposal submission
through the IGBB. Any IGBB Faculty Affiliate that submits and receives an external grant (with
overhead) through the IGBB will be eligible for the IGBB Fellows Program.

A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes
IGBB Authors:
Mark L. LawrencePUBLICATION YEAR:
2007IMPACT FACTOR:
2.893CITATION COUNT:
209Liu D, Lawrence ML, Austin FW, Ainsworth AJ (2007) A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes.
Journal of Microbiological Methods 71(2): 133-140.
DOI:
10.1016/j.mimet.2007.08.007EID:
2-s2.0-35148830364PMID: 17884210
DOWNLOAD PDFABSTRACTListeria monocytogenes internalin gene inlJ has been described previously for differentiation of virulent from avirulent strains. However, a recent report indicated that there exist some unusual lineage IIIB strains (e.g., serotype 7 strain R2-142) that possess no inlJ gene but have the capacity to cause mouse mortality via intraperitoneal inoculation. Therefore, a multiplex PCR incorporating inlA, inlC and inlJ gene primers was developed in this study for rapid speciation and virulence determination of L. monocytogenes. Although inlB gene was also assessed for species-specific recognition, it was not included in the multiplex PCR due to the negative reaction observed between the inlB primers and serotypes 4a-e strains. The species identity of the 36 L. monocytogenes strains under investigation was verified through the amplification of an 800 bp fragment with the inlA primers and the virulence of these strains was ascertained by the formation of 517 bp and/or 238 bp fragments with the inlC and inlJ primers, respectively. Whereas L. monocytogenes pathogenic strains with capacity to cause mortality (showing relative virulence of 30-100%) in A/J mice via the intraperitoneal route were invariably detected by the inlC and/or inlJ primers, naturally non-pathogenic strains (showing relative virulence of 0%) were negative with these primers. While 8 of the 10 L. ivanovii strains reacted with the inlC primers, they could be effectively excluded as non-L. monocytogenes through their negative reactions with the inlA primers in the multiplex PCR. Thus, the use of the multiplex PCR targeting inlA, inlC and inlJ genes facilitates simultaneous confirmation of L. monocytogenes species identity and virulence.
Dr. Matthew W. 
BrownAssociate Professor
IGBB Fellow
website (662) 325-2406
Dr. Bindu Nanduri
Professor
IGBB Fellow
(662) 325-4217
Dr. Zhaohua Peng
Professor
IGBB Fellow
(662) 325-0685
Dr. Hossam Abdelhamed
Assistant Research Professor
IGBB Affiliate
(662) 325-1125
Dr. Pratima 
Acharya AdhikariAssistant Professor
IGBB Affiliate
website (662) 325-3416
Dr. Peter J. Allen
Professor
IGBB Affiliate
(662) 325-4768
Dr. Ashli Brown
Professor
IGBB Affiliate
(662) 325-3324
Dr. Sam Chang
Professor
IGBB Affiliate
(662) 325-3200
Dr. Wen-Hsing Cheng
Professor
IGBB Affiliate
(662) 325-3200
Dr. Sandra B. 
CorreaAssistant Professor
IGBB Affiliate
website (662) 325-0158
Dr. Angus Dawe
Professor
IGBB Affiliate
(662) 325-7577
Dr. Thu 
DinhAssociate Professor
IGBB Affiliate
website (662) 325-7554
Dr. Peixin Fan
Assistant Professor
IGBB Affiliate
(662) 325-0963
Dr. Jean M.N. Feugang
Associate Research Professor
IGBB Affiliate
(662) 325-7567
Dr. Christine Fortuin
Assistant Professor
IGBB Affiliate
(662) 325-0282
Dr. Esteban Galeano
Assistant Professor
IGBB Affiliate
(662) 325-7782
Dr. Austin Himes
Assistant Professor
IGBB Affiliate
(662) 325-4249
Dr. Federico G. 
HoffmannAssociate Professor
IGBB Affiliate
website (662) 325-2763
Dr. Attila Karsi
Professor
IGBB Affiliate
(662) 325-0405
Dr. Margaret L. Khaitsa
Professor
IGBB Affiliate
(662) 325-1365
Dr. William Kingery
Professor
IGBB Affiliate
(662) 325-2311
Dr. Andrew 
KoubaProfessor
IGBB Affiliate
website (662) 325-2378
Dr. Natraj (Krish) Krishnan
Associate Professor
IGBB Affiliate
(662) 325-2978
Dr. Mark Lawrence
Professor
IGBB Affiliate
(662) 325-1205
Dr. Caleb Lemley
Associate Professor
IGBB Affiliate
(662) 325-2934
Dr. Shien Lu
Professor
IGBB Affiliate
(662) 325-3511
Dr. Benjamin 
MagbanuaAssociate Professor
IGBB Affiliate
website (662) 325-9777
Dr. Florencia Meyer
Associate Professor
IGBB Affiliate
(662) 325-7734
Dr. Ramakrishna (Rama) Nannapaneni
Research Professor
IGBB Affiliate
(662) 325-7697
Dr. Diana Outlaw
Associate Professor
IGBB Affiliate
(662) 325-4803
Dr. E. David Peebles
Professor
IGBB Affiliate
(662) 325-3379
Dr. Andy 
PerkinsProfessor
IGBB Affiliate
website (662) 325-0004
Dr. Sorina Popescu
Associate Professor
IGBB Affiliate
(662) 325-7735
Dr. Lauren 
PriddyAssistant Professor
IGBB Affiliate
website (662) 325-5988
Dr. Stephen B. Pruett
Professor
IGBB Affiliate
(662) 325-6653
Dr. K. Raja 
ReddyResearch Professor
IGBB Affiliate
website (662) 325-9463
Dr. John 
RigginsProfessor
IGBB Affiliate
website (662) 325-2984
Dr. Leyla 
Rios de AlvarezAssistant Professor
IGBB Affiliate
website (662) 325-2802
Dr. Sejo Sabanadzovic
Professor
IGBB Affiliate
(662) 325-9322
Dr. Michael Sandel
Assistant Professor
IGBB Affiliate
(662) 325-3133
Dr. Wes Schilling
Professor
IGBB Affiliate
(662) 325-9456
Dr. Keun-Seok Seo
Associate Professor
IGBB Affiliate
(662) 325-1419
Dr. Xueyan Shan
Associate Research Professor
IGBB Affiliate
(662) 325-2640
Dr. Rubin Shmulsky
Professor
IGBB Affiliate
(662) 325-2243
Dr. Juan L. 
SilvaProfessor
IGBB Affiliate
website (662) 325-3200
Dr. C. Beth Stokes
Associate Professor
IGBB Affiliate
(662) 325-5811
Dr. Justin 
ThorntonAssociate Professor
IGBB Affiliate
website (662) 325-8020
Dr. Te-Ming (Paul) Tseng
Associate Professor
IGBB Affiliate
(662) 325-4725
Dr. Carrie 
VanceAssociate Research Professor
IGBB Affiliate
website (662) 325-2640
Dr. Guiming Wang
Professor
IGBB Affiliate
(662) 325-0414
Dr. Mark 
WelchProfessor
IGBB Affiliate
website (662) 325-7564
Dr. Li 
ZhangAssistant Research Professor
IGBB Affiliate
website (662) 325-3416
Dr. Xue 
ZhangAssistant Research Professor
IGBB Affiliate
website (662) 325-3200
Former Fellows & Affiliates (Position with IGBB; Current Institution)
Jeffrey F.D. Dean (Affiliate; University of Georgia)
Jonas King (Affiliate)
Hongxu Dong (Affiliate; Goldman Sachs, Atlanta)
Terezie Tolar-Peterson (Affiliate; California State University, San Bernadino)
Erdogan Memili (Affiliate; Prairie View A&M University)
Gregory Bohach (Affiliate; retired)
Brian A. Counterman (Affiliate; Auburn University)
Renita Horton (Affiliate; University of Houston)
Xiu-feng "Henry" Wan (Affiliate; University of Missouri)
Din-Pow Ma (Affiliate; retired)
David A. Ray (Fellow; Texas Tech University)
Shane C. Burgess (Fellow & former IGBB Director; University of Arizona)
Fiona M. McCarthy (Fellow; University of Arizona)
Susan M. Bridges (Fellow; retired)
Susan V. Diehl (Affiliate; retired)
James N. Warnock (Affiliate; University of Georgia)
Dragica Jeremic Nikolic (Affiliate; University of Toronto)
Cetin Yuceer (Affiliate; Turkey)
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.