All Fields Required Unless Otherwise Stated
After registering, your account on the IGBB system will be ready to use. By default, you will be identified as an external user (non-MSU Employee).
If you are an MSU employee and you are interested in purchasing services from IGBB, your account type can be updated by IGBB personnel.
Topic:
Sequencing the rohu carp genome
IGBB Scientists:
Chuan-Yu Hsu
Tony Arick
Daniel Peterson
Funding:
USAID

A 5-methylcytosine DNA glycosylase/lyase demethylates the retrotransposon Tos17 and promotes its transposition in rice
IGBB Authors:
Zhaohua PengPUBLICATION YEAR:
2011IMPACT FACTOR:
10.425CITATION COUNT:
84La H, Ding B, Mishra GP, Zhou B, Yang H, Bellizzi Mdel R, Chen S, Meyers BC, Peng Z, Zhu JK,Wang GL (2011) A 5-methylcytosine DNA glycosylase/lyase demethylates the retrotransposon Tos17 and promotes its transposition in rice.
Proceedings of the National Academy of Sciences of the United States of America 108(37): 15498-15503.
DOI:
10.1073/pnas.1112704108EID:
2-s2.0-80053065324PMID: 21896764
DOWNLOAD PDFABSTRACTDNA 5-methylcytosine (5-meC) is an important epigenetic mark for transcriptional gene silencing in many eukaryotes. In Arabidopsis, 5-meC DNA glycosylase/lyases actively remove 5-meC to counteract transcriptional gene silencing in a locus-specific manner, and have been suggested to maintain the expression of transposons. However, it is unclear whether plant DNA demethylases can promote the transposition of transposons. Here we report the functional characterization of the DNA glycosylase/lyase DNG701 in rice. DNG701 encodes a large (1,812 amino acid residues) DNA glycosylase domain protein. Recombinant DNG701 protein showed 5-meC DNA glycosylase and lyase activities in vitro. Knockout or knockdown of DNG701 in rice plants led to DNA hypermethylation and reduced expression of the retrotransposon Tos17. Tos17 showed less transposition in calli derived from dng701 knockout mutant seeds compared with that in wild-type calli. Overexpression of DNG701 in both rice calli and transgenic plants substantially reduced DNA methylation levels of Tos17 and enhanced its expression. The overexpression also led to more frequent transposition of Tos17 in calli. Our results demonstrate that rice DNG701 is a 5-meC DNA glycosylase/lyase responsible for the demethylation of Tos17 and this DNA demethylase plays a critical role in promoting Tos17 transposition in rice calli.


Dr. E. David PeeblesProfessor
Poultry ScienceIGBB Affiliate
email(662) 325-3379
Dr. Justin ThorntonAssociate Professor
Biological SciencesIGBB Affiliate
email(662) 325-8020
website
Dr. Chuan-Yu Hsu (Shu or Sue)Senior Research Associate
GENOMICS LEAD
email(662) 325-9511
Pace 121
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.