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Topic:
Cotton improvement
IGBB Scientists:
Daniel Peterson
Tony Arick
Chuan-Yu Hsu
Funding:
USDA

Reprogramming mammalian somatic cells
IGBB Authors:
Erdogan Memili, Nelida Rodriguez-OsorioPUBLICATION YEAR:
2012IMPACT FACTOR:
2.24CITATION COUNT:
69Rodriguez-Osorio N, Urrego R, Cibelli JB, Eilertsen K, Memili E (2012) Reprogramming mammalian somatic cells.
Theriogenology 78(9): 1869-1886.
DOI:
10.1016/j.theriogenology.2012.05.030EID:
2-s2.0-84868199540PMID: 22979962
DOWNLOAD PDFABSTRACTSomatic cell nuclear transfer (SCNT), the technique commonly known as cloning, permits transformation of a somatic cell into an undifferentiated zygote with the potential to develop into a newborn animal (i.e., a clone). In somatic cells, chromatin is programmed to repress most genes and express some, depending on the tissue. It is evident that the enucleated oocyte provides the environment in which embryonic genes in a somatic cell can be expressed. This process is controlled by a series of epigenetic modifications, generally referred to as "nuclear reprogramming," which are thought to involve the removal of reversible epigenetic changes acquired during cell differentiation. A similar process is thought to occur by overexpression of key transcription factors to generate induced pluripotent stem cells (iPSCs), bypassing the need for SCNT. Despite its obvious scientific and medical importance, and the great number of studies addressing the subject, the molecular basis of reprogramming in both reprogramming strategies is largely unknown. The present review focuses on the cellular and molecular events that occur during nuclear reprogramming in the context of SCNT and the various approaches currently being used to improve nuclear reprogramming. A better understanding of the reprogramming mechanism will have a direct impact on the efficiency of current SCNT procedures, as well as iPSC derivation.


Dr. Peter J. AllenAssociate Professor
Wildlife, Fisheries & AquacultureIGBB Affiliate
email(662) 325-4768
Dr. Wen-Hsing ChengProfessor
Food Science, Nutrition & Health PromotionIGBB Affiliate
email(662) 325-3200
Mark A. Arick, II (Tony)Computer Specialist
BIOCOMPUTING LEAD
email(662) 325-0583
Pace 110
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.