Gel-Based Protein Isolation, Labeling & Imaging


P2000A
Isoelectric focusing (IEF) 11 cm strip; first strip
Strip
$42.57

Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).

P2050A
Isoelectric focusing (IEF) 11 cm strip; additional strips up to 12
Strip
$21.81

Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).

P2100A
Isoelectric focusing (IEF) 17 cm strip; first strip
Strip
$44.41

Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).

P2150A
Isoelectric focusing (IEF) 17 cm strip; additional strips up to 12
Strip
$23.64

Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).

P2200A
2D PAGE on slab gel; first gel
Gel
$109.04

Customer provides precast gel of their choice.

P2250A
2D PAGE on slab gel; second gel
Gel
$24.88

Customer provides precast gel(s) of their choice.

P2300A
Gel staining with Coomassie Blue
Gel
$21.40

P2350A
Gel staining with Sypro Ruby
Gel
$90.44

P2400A
Visual and UV protein gel imaging
Gel
$20.44

P2450A
Gel analysis with PD Quest software
Hour
$52.14

P2500A
CyDye protein labeling for 2D DIGE, 1st dye
Sample
$280.42

Customer must provide quantified protein sample dissolved in CyDye compatible buffer (ask IGBB staff for details). Sample will be labeled with customer's choice of Cy3, Cy5, or Cy2.

P2550A
CyDye protein labeling for 2D DIGE, 2nd dye
Sample
$198.91

Customer must provide quantified protein sample dissolved in CyDye compatible buffer (ask IGBB staff for details). Sample will be labeled with customer's choice of Cy3, Cy5, or Cy2.

P2600A
CyDye protein labeling for 2D DIGE, 3rd dye
Sample
$198.91

Customer must provide quantified protein sample dissolved in CyDye compatible buffer (ask IGBB staff for details). Sample will be labeled with customer's choice of Cy3, Cy5, or Cy2.

P2650A
CyDye stained gel imaging
Hour
$110.10

Reagents, plasticware, etc., are the responsibility of the customer.

P2700A
DIGE gel analysis using DeCyder software
Hour
$52.14

IGBB staff will conduct the analysis and discuss the results with the customer.

P2750A
Manual sample spotting (for MALDI mass spectrometric analysis)
Spot
$5.59

Performed by IGBB staff if samples are not provided on an ABI4700 MALDI plate.

P2800A
Spot/band excision from gel (minimum 20 spots/bands required)
Spot/band
$16.48

Gel plugs will be deposited into 96 well plate(s).

P2850A
In-gel digestion, desalting, and spotting (minimum 20 spots/bands required)
Spot/band
$26.05

Peptides will be spotted on an ABI4700 MALDI plate(s).

P2900A
WES capillary-based immunoassay (minimum 20 wells/run)
Well
$27.58

Customer should provide quantitated protein sample and primary antibody.

The IGBB is supported, in part, by the following units:


The IGBB is an HPC² member center.