Gel-Based Protein Isolation, Labeling & Imaging
Cat. No.
Service
Unit
Internal User Cost
Service:
Isoelectric focusing (IEF) 11 cm strip; first strip
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
Isoelectric focusing (IEF) 11 cm strip; additional strips up to 12
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
Isoelectric focusing (IEF) 17 cm strip; first strip
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
Isoelectric focusing (IEF) 17 cm strip; additional strips up to 12
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
2D PAGE on slab gel; first gel
Customer provides precast gel of their choice.
Service:
2D PAGE on slab gel; second gel
Customer provides precast gel(s) of their choice.
Service:
Gel staining with Coomassie Blue
Service:
Gel staining with Sypro Ruby
Service:
Visual and UV protein gel imaging
Service:
Gel analysis with PD Quest software
Service:
CyDye protein labeling for 2D DIGE, 1st dye
Customer must provide quantified protein sample dissolved in CyDye compatible buffer (ask IGBB staff for details). Sample will be labeled with customer's choice of Cy3, Cy5, or Cy2.
Service:
CyDye protein labeling for 2D DIGE, 2nd dye
Customer must provide quantified protein sample dissolved in CyDye compatible buffer (ask IGBB staff for details). Sample will be labeled with customer's choice of Cy3, Cy5, or Cy2.
Service:
CyDye protein labeling for 2D DIGE, 3rd dye
Customer must provide quantified protein sample dissolved in CyDye compatible buffer (ask IGBB staff for details). Sample will be labeled with customer's choice of Cy3, Cy5, or Cy2.
Service:
CyDye stained gel imaging
Reagents, plasticware, etc., are the responsibility of the customer.
Service:
DIGE gel analysis using DeCyder software
IGBB staff will conduct the analysis and discuss the results with the customer.
Service:
Manual sample spotting (for MALDI mass spectrometric analysis)
Performed by IGBB staff if samples are not provided on an ABI4700 MALDI plate.
Service:
Spot/band excision from gel (minimum 20 spots/bands required)
Gel plugs will be deposited into 96 well plate(s).
Service:
In-gel digestion, desalting, and spotting (minimum 20 spots/bands required)
Peptides will be spotted on an ABI4700 MALDI plate(s).
Service:
WES capillary-based immunoassay (minimum 20 wells/run)
Customer should provide quantitated protein sample and primary antibody.
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.