Gel-Based Protein Isolation, Labeling & Imaging
Cat. No.
Service
Unit
Internal User Cost
Service:
Isoelectric focusing (IEF) 11 cm strip; first strip
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
Isoelectric focusing (IEF) 11 cm strip; additional strips up to 12
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
Isoelectric focusing (IEF) 17 cm strip; first strip
Customer must provide quantified protein sample dissolved in IEF compatible buffer (ask IGBB staff for details).
Service:
2D PAGE on slab gel; first gel
Customer provides precast gel of their choice.
Service:
2D PAGE on slab gel; second gel
Customer provides precast gel(s) of their choice.
Service:
Gel staining with Coomassie Blue
Service:
Gel staining with Oreole
Service:
Visual and UV protein gel imaging
Service:
Gel analysis with PD Quest software
Service:
CyDye stained gel imaging
Reagents, plasticware, etc., are the responsibility of the customer.
Service:
DIGE gel analysis using DeCyder software
IGBB staff will conduct the analysis and discuss the results with the customer.
Service:
Spot/band excision from gel (minimum 20 spots/bands required)
Gel plugs will be deposited into 96 well plate(s).
Service:
In-gel digestion, desalting, and spotting (minimum 20 spots/bands required)
Peptides will be spotted on an ABI4700 MALDI plate(s).
Service:
WES capillary-based immunoassay (minimum 20 wells/run)
Customer should provide quantitated protein sample and primary antibody.
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.