Illumina Library Prep & Sequencing
Cat. No.
Service
Unit
Internal User Cost
Service:
Low input directional mRNA library with rRNA removal by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Low input directional mRNA library with rRNA removal by Novogene; each additional library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Illumina library purification by Novogene
This service is for those who have made their own DNA libraries and wish Novogene to do standard clean-up of the aforementioned libraries.
Service:
Ultra low input cDNA library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Ultra low input cDNA library by Novogene; each additional library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) PCR-free library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) PCR-free library construction by Novogene; each additional library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Eukaryotic directional mRNA library construction (NEB) by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Eukaryotic directional mRNA library construction (NEB) by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Prokaryotic/LncRNA/FFPE/total RNA library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Prokaryotic/LncRNA/FFPE/total RNA library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Small RNA library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Small RNA library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
ChIP-Seq library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
ChIP-Seq library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metagenomic library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metagenomic library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metatranscriptomic library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metatranscriptomic library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction and sequencing (PE250) with 1M raw reads by Novo
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction and sequencing (PE250) with 1M raw reads by Novo
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 30K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 30K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 50K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 50K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 100K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 100K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Whole Genome Bisulfite Sequencing (WGBS) library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Whole Genome Bisulfite Sequencing (WGBS) library construction by Novogene; each additional library u
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metagenomic 16S amplicon library prep
Customer provides at least 1 ug genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing.
Service:
Double-digest RADseq library preparation for genotyping
Double-digest RADseq (ddRADseq) is a reduced-representation technique that allows scientists to produce genotypes for a given organism without the prerequisite of a whole genome sequence. For information contact the IGBB's Genomics Lead.
Service:
NovaSeq S4 (PE150) sequencing by Novogene; cost per gigabase
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
NovaSeq S4 RNAseq (PE150) sequencing by Novogene if library/libraries made by Novogene; 12 gigabases
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
NovaSeq S4 Flow Cell (PE150; 800 Gb raw data) full lane sequencing by Novogene
Unit Cost (USD):
$6,078.24
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
Service:
NovaSeq S4 Flow Cell (PE150) partial lane sequencing by Novogene; 30Gb ≤ X ≤60Gb
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
NovaSeq S4 Flow Cell (PE150) partial lane sequencing by Novogene; 60Gb < X ≤ 100Gb
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
NovaSeq S4 Flow Cell (PE150) partial lane sequencing by Novogene; 100Gb < X ≤ 400Gb
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
NovaSeq S4 Flow Cell (PE150) partial lane sequencing by Novogene; 400Gb < X
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
NovaSeq 6000 SP (PE250; ~ 400 M raw reads/lane) full lane sequencing by Novogene
Unit Cost (USD):
$5,129.24
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
Service:
NovaSeq 6000 SP (PE50/SE50; ~ 400 M raw reads/lane) full lane sequencing by Novogene
Unit Cost (USD):
$2,129.24
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
Service:
NovaSeq X Plus (PE150; 375 Gb raw data/lane) full lane sequencing by Novogene
Unit Cost (USD):
$2,228.24
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.