Illumina Library Prep & Sequencing
Cat. No.
Service
Unit
Internal User Cost
Service:
Low input directional mRNA library with rRNA removal by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Low input directional mRNA library with rRNA removal by Novogene; each additional library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Illumina library purification by Novogene
This service is for those who have made their own DNA libraries and wish Novogene to do standard clean-up of the aforementioned libraries.
Service:
Ultra low input cDNA library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Ultra low input cDNA library by Novogene; each additional library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) PCR-free library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
DNA short-insert (500 bp or less) PCR-free library construction by Novogene; each additional library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Eukaryotic directional mRNA library construction (NEB) by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Eukaryotic directional mRNA library construction (NEB) by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Prokaryotic/LncRNA/FFPE/total RNA library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Prokaryotic/LncRNA/FFPE/total RNA library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Small RNA library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Small RNA library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
ChIP-Seq library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
ChIP-Seq library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metagenomic library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metagenomic library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metatranscriptomic library construction by Novogene; first library
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metatranscriptomic library construction by Novogene; each additional library up to 25
For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction and sequencing (PE250) with 1M raw reads by Novo
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction and sequencing (PE250) with 1M raw reads by Novo
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 30K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 30K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 50K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 50K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 100K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 100K
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Whole Genome Bisulfite Sequencing (WGBS) library construction by Novogene; first library
For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Whole Genome Bisulfite Sequencing (WGBS) library construction by Novogene; each additional library u
Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.
Service:
Metagenomic 16S amplicon library prep
Customer providesat least 1 ug genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing.
Service:
Double-digest RADseq library preparation for genotyping
Double-digest RADseq (ddRADseq) is a reduced-representation technique that allows scientists to produce genotypes for a given organism without the prerequisite of a whole genome sequence. For information contact the IGBB's Genomics Lead.
Service:
MiSeq (1x150 bp or PE75)
Unit Cost (USD):
$1,437.73
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Unit Cost (USD):
$2,132.73
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Unit Cost (USD):
$2,132.73
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Unit Cost (USD):
$2,132.73
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Unit Cost (USD):
$2,132.73
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Service:
MiSeq up to 300-cycle run where MiSeq reagent kit is provided by customer
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Service:
MiSeq greater than 300-cycle run where MiSeq reagent kit is provided by customer
A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.
Service:
Illumina (PE150) by Novogene; cost per gigabase
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
Illumina RNAseq (PE150) by Novogene if library/libraries made by Novogene; 12 gigabases
Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.
Service:
Illumina (PE150) by Novogene if library/libraries not made by Novogene
Unit Cost (USD):
$1,863.14
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
Service:
Illumina (PE150) by Novogene if library/libraries made by Novogene
Unit Cost (USD):
$1,587.21
A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.
Service:
Illumina (1x50 bp) by Novogene if library/libraries made by Novogene; cost per million reads
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
Service:
NovaSeq S4 Flow Cell (PE150; 800 Gb raw data) by Novogene if library/libraries made by Novogene
Unit Cost (USD):
$6,064.47
If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.
Service:
NovaSeq S4 Flow Cell (PE150; 800 Gb raw data) by Novogene if library/libraries not made by Novogene
Unit Cost (USD):
$6,088.55
A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.
Service:
NovaSeq S4 Flow Cell (1x50 bp; 800 M reads) by Novogene if library/libraries made by Novogene
Unit Cost (USD):
$3,115.47
A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.
Service:
NovaSeq S4 Flow Cell (1x50 bp; 1600 M reads) by Novogene if library/libraries made by Novogene
Unit Cost (USD):
$6,115.47
A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.
Service:
NovaSeq S4 Flow Cell (1x50 bp; 800 M reads) by Novogene if library/libraries not made by Novogene
Unit Cost (USD):
$3,139.55
A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.
Service:
NovaSeq S4 Flow Cell (1x50 bp; 1600 M reads) by Novogene if library/libraries not made by Novogene
Unit Cost (USD):
$6,139.55
A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.