Illumina Library Prep & Sequencing


G4223B
Low input directional mRNA library with rRNA removal by Novogene; first library
Library
$334.88

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4224B
Low input directional mRNA library with rRNA removal by Novogene; each additional library
Library
$310.80

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4225B
Illumina library purification by Novogene
Sample
$47.22

This service is for those who have made their own DNA libraries and wish Novogene to do standard clean-up of the aforementioned libraries.

G4226B
Ultra low input cDNA library construction by Novogene; first library
Library
$334.88

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4227B
Ultra low input cDNA library by Novogene; each additional library
Library
$310.80

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4230B
DNA short-insert (500 bp or less) library construction by Novogene; first library
Library
$134.88

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4231B
DNA short-insert (500 bp or less) library construction by Novogene; each additional library up to 25
Library
$110.80

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4232B
DNA short-insert (500 bp or less) PCR-free library construction by Novogene; first library
Library
$174.88

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4233B
DNA short-insert (500 bp or less) PCR-free library construction by Novogene; each additional library
Library
$150.80

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4234B
Eukaryotic directional mRNA library construction (NEB) by Novogene; first library
Library
$164.88

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4235B
Eukaryotic directional mRNA library construction (NEB) by Novogene; each additional library up to 25
Library
$140.80

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4238B
Prokaryotic/LncRNA/FFPE/total RNA library construction by Novogene; first library
Library
$334.88

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4239B
Prokaryotic/LncRNA/FFPE/total RNA library construction by Novogene; each additional library up to 25
Library
$310.80

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4240B
Small RNA library construction by Novogene; first library
Library
$314.88

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4241B
Small RNA library construction by Novogene; each additional library up to 25
Library
$290.80

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4244B
ChIP-Seq library construction by Novogene; first library
Library
$134.88

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4245B
ChIP-Seq library construction by Novogene; each additional library up to 25
Library
$110.80

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4246B
Metagenomic library construction by Novogene; first library
Library
$134.88

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4247B
Metagenomic library construction by Novogene; each additional library up to 25
Library
$110.80

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4248B
Metatranscriptomic library construction by Novogene; first library
Library
$294.88

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4249B
Metatranscriptomic library construction by Novogene; each additional library up to 25
Library
$270.80

For Illumina sequencing. Customer provides 0.5-4 ug total RNA in 10-30 ul nuclease-free water or 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4250B
Microbial 16S/18S/ITS amplicon library construction and sequencing (PE250) with 1M raw reads by Novo
Library
$142.88

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4251B
Microbial 16S/18S/ITS amplicon library construction and sequencing (PE250) with 1M raw reads by Novo
Library
$118.80

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4252B
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 30K
Library
$74.88

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4253B
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 30K
Library
$50.80

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4254B
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 50K
Library
$89.88

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4255B
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 50K
Library
$65.80

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4256B
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 100K
Library
$104.88

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4257B
Microbial 16S/18S/ITS amplicon library construction, sequencing (PE250), and data analysis with 100K
Library
$80.80

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4258B
Whole Genome Bisulfite Sequencing (WGBS) library construction by Novogene; first library
Library
$334.88

For Illumina sequencing. Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4259B
Whole Genome Bisulfite Sequencing (WGBS) library construction by Novogene; each additional library u
Library
$310.80

Customer provides at least 2 ug high molecular weight genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing. If requested, a barcode adaptor will be added to fragments used in library construction.

G4260B
Metagenomic 16S amplicon library prep
Sample
$21.80

Customer providesat least 1 ug genomic DNA in 20-50 ul 10 mM Tris buffer (pH 8.5). Samples that do not meet minimum standards will be returned without further processing.

G4400B
Double-digest RADseq library preparation for genotyping
Sample
$121.65

Double-digest RADseq (ddRADseq) is a reduced-representation technique that allows scientists to produce genotypes for a given organism without the prerequisite of a whole genome sequence. For information contact the IGBB's Genomics Lead.

G4600B
MiSeq (1x150 bp or PE75)
Flow-cell lane
$1,437.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4650B
MiSeq (PE150)
Flow-cell lane
$2,132.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4700B
MiSeq (PE200)
Flow-cell lane
$2,132.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4750B
MiSeq (PE250)
Flow-cell lane
$2,132.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4800B
MiSeq (PE300)
Flow-cell lane
$2,132.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4850B
MiSeq up to 300-cycle run where MiSeq reagent kit is provided by customer
Flow-cell lane
$427.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4900B
MiSeq greater than 300-cycle run where MiSeq reagent kit is provided by customer
Flow-cell lane
$427.73

A minimum sequence output of 10 million reads per lane (average) is guaranteed if library is prepared by IGBB. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by the IGBB.

G4910B
Illumina (PE150) by Novogene; cost per gigabase
Gbp
$34.04

Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.

G4911B
Illumina RNAseq (PE150) by Novogene if library/libraries made by Novogene; 12 gigabases
12 Gbp
$232.04

Novogene only provides per Gb sequencing if it has made the Illumina library being sequenced.

G4912B
Illumina (PE150) by Novogene if library/libraries not made by Novogene
100 Gbp
$1,863.14

If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.

G4913B
Illumina (PE150) by Novogene if library/libraries made by Novogene
100 Gbp
$1,587.21

A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.

G4914B
Illumina (1x50 bp) by Novogene if library/libraries made by Novogene; cost per million reads
1 M reads
$36.04

If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.

G4930B
NovaSeq S4 Flow Cell (PE150; 800 Gb raw data) by Novogene if library/libraries made by Novogene
Flow-cell lane
$6,064.47

If customers have had their library/libraries made by Novogene specifically for sequencing by Novogene, there is less work required of IGBB employees. This is reflected in the price.

G4931B
NovaSeq S4 Flow Cell (PE150; 800 Gb raw data) by Novogene if library/libraries not made by Novogene
Flow-cell lane
$6,088.55

A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.

G4932B
NovaSeq S4 Flow Cell (1x50 bp; 800 M reads) by Novogene if library/libraries made by Novogene
Flow-cell lane
$3,115.47

A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.

G4933B
NovaSeq S4 Flow Cell (1x50 bp; 1600 M reads) by Novogene if library/libraries made by Novogene
2 FC lanes
$6,115.47

A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.

G4935B
NovaSeq S4 Flow Cell (1x50 bp; 800 M reads) by Novogene if library/libraries not made by Novogene
Flow-cell lane
$3,139.55

A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.

G4936B
NovaSeq S4 Flow Cell (1x50 bp; 1600 M reads) by Novogene if library/libraries not made by Novogene
2 FC lanes
$6,139.55

A minimum sequence output of greater than or equal to 100 million reads per lane (average) is guaranteed if library is prepared by Novogene. If the customer provides a library made elsewhere, the library should be at a concentration of 10 nm (minimum volume of 10 ul) in 10 mM Tris buffer (pH 8.5). There is no guarantee on reads per lane if the library is not generated by Novogene.

The IGBB is supported, in part, by the following units:


The IGBB is an HPC² member center.