While the IGBB Service Center can perform low-risk, fairly routine biomolecular tasks on a fee-per-service basis, some of your research goals may require (or would most readily be completed with) the help of professionals trained in advanced procedures and/or data analysis techniques. The IGBB research associates, most of whom have Ph.D. degrees and/or many years of practical experience, can be hired to conduct research tasks associated with their specialties. For example, let's say you need an expert in RNA isolation and RNAseq to help you complete a portion of a research project. You could train a student or postdoc to become proficient in these research tasks, but you may not have the experience, time, or money to train someone. Moreover, you may not have the instrumentation you need to do the research correctly. This is where the IGBB can help. Rather than training someone new, you can hire a highly skilled IGBB scientist to perform the research for you. The savings in cost and time is tremendous! The process works like this:
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If you don't have funding in hand, but are working on a grant proposal, you can include the participation of an IGBB expert-for-hire in your proposal budget (note: you must make such agreements with the expert-for-hire through a IGBB itemized quote). To discuss a hiring an IGBB expert, please contact one of the IGBB's Research Leads by phone or e-mail.
NOTE: PIs are asked to consider whether the participation of an IGBB employee in a project merits that employee's inclusion as a co-author on a resulting manuscript(s). The decision ultimately lies with the PI. However, the IGBB encourages IGBB staff and faculty involved in
Proposal Partnerships and
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Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos
IGBB Authors:
Erdogan MemiliPUBLICATION YEAR:
2006IMPACT FACTOR:
10.977CITATION COUNT:
140Misirlioglu M, Page GP, Sagirkaya H, Kaya A, Parrish JJ, First NL, Memili E (2006) Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos.
Proceedings of the National Academy of Sciences of the United States of America 103(50): 18905-18910.
DOI:
10.1073/pnas.0608247103EID:
2-s2.0-34248651844PMID: 17142320
DOWNLOAD PDFABSTRACTGlobal activation of the embryonic genome is the most critical event in early mammalian development. After fertilization, a rich supply of maternal proteins and RNAs support development whereas a number of zygotic and embryonic genes are expressed in a stage-specific manner leading to embryonic genome activation (EGA). However, the identities of embryonic genes expressed and the mechanism(s) of EGA are poorly defined in the bovine. Using the Affymetrix bovine-specific DNA microarray as the biggest available array at present, we analyzed gene expression at two key stages of bovine development, matured oocytes (MII) and 8-cell-stage embryos, constituting the ultimate reservoir for life and a stage during which EGA takes place, respectively. Key genes in regulation of transcription, chromatin-structure cell adhesion, and signal transduction were up-regulated at the 8-cell stage as compared with 8-cell embryos treated with alpha-amanitin and MII. Genes controlling DNA methylation and metabolism were up-regulated in MII. These changes in gene expression, related to transcriptional machinery, chromatin structure, and the other cellular functions occurring during several cleavage stages, are expected to result in a unique chromatin structure capable of maintaining totipotency during embryogenesis and leading to differentiation during postimplantation development. Dramatic reprogramming of gene expression at the onset of development also has implications for cell plasticity in somatic cell nuclear transfer, genomic imprinting, and cancer.
Mark A. Arick, II (Tony)Interm Director & Computer Specialist
BIOCOMPUTING LEAD
email(662) 325-0583
Pace 110
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