While the IGBB Service Center can perform low-risk, fairly routine biomolecular tasks on a fee-per-service basis, some of your research goals may require (or would most readily be completed with) the help of professionals trained in advanced procedures and/or data analysis techniques. The IGBB research associates, most of whom have Ph.D. degrees and/or many years of practical experience, can be hired to conduct research tasks associated with their specialties. For example, let's say you need an expert in RNA isolation and RNAseq to help you complete a portion of a research project. You could train a student or postdoc to become proficient in these research tasks, but you may not have the experience, time, or money to train someone. Moreover, you may not have the instrumentation you need to do the research correctly. This is where the IGBB can help. Rather than training someone new, you can hire a highly skilled IGBB scientist to perform the research for you. The savings in cost and time is tremendous! The process works like this:
- The principal investigator (PIs) interested in determining whether
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If you don't have funding in hand, but are working on a grant proposal, you can include the participation of an IGBB expert-for-hire in your proposal budget (note: you must make such agreements with the expert-for-hire through a MyIGBB itemized quote). To discuss a hiring an IGBB expert, please contact one of the IGBB's Research Leads by phone or e-mail.
NOTE: PIs are asked to consider whether the participation of an IGBB employee in a project merits that employee's inclusion as a co-author on a resulting manuscript(s). The decision ultimately lies with the PI. However, the IGBB encourages IGBB staff and faculty involved in
Proposal Partnerships and
Experts for Hire to discuss/negotiate co-authorship with PIs before starting work on a project.

ProtQuant: a tool for the label-free quantification of MudPIT proteomics data
IGBB Authors:
Susan M. Bridges, Shane C. Burgess, Bindu NanduriPUBLICATION YEAR:
2007IMPACT FACTOR:
4.249CITATION COUNT:
46Bridges SM, Magee GB, Wang N, Williams WP, Burgess SC, Nanduri B (2007) ProtQuant: a tool for the label-free quantification of MudPIT proteomics data.
BMC Bioinformatics 8(Suppl 7): S24.
DOI:
10.1186/1471-2105-8-S7-S24EID:
2-s2.0-38549091691PMID: 18047724
DOWNLOAD PDFABSTRACTBACKGROUND: Effective and economical methods for quantitative analysis of high throughput mass spectrometry data are essential to meet the goals of directly identifying, characterizing, and quantifying proteins from a particular cell state. Multidimensional Protein Identification Technology (MudPIT) is a common approach used in protein identification. Two types of methods are used to detect differential protein expression in MudPIT experiments: those involving stable isotope labelling and the so-called label-free methods. Label-free methods are based on the relationship between protein abundance and sampling statistics such as peptide count, spectral count, probabilistic peptide identification scores, and sum of peptide Sequest XCorr scores (SigmaXCorr). Although a number of label-free methods for protein quantification have been described in the literature, there are few publicly available tools that implement these methods. We describe ProtQuant, a Java-based tool for label-free protein quantification that uses the previously published SigmaXCorr method for quantification and includes an improved method for handling missing data. RESULTS: ProtQuant was designed for ease of use and portability for the bench scientist. It implements the SigmaXCorr method for label free protein quantification from MudPIT datasets. ProtQuant has a graphical user interface, accepts multiple file formats, is not limited by the size of the input files, and can process any number of replicates and any number of treatments. In addition, ProtQuant implements a new method for dealing with missing values for peptide scores used for quantification. The new algorithm, called SigmaXCorr.
Dr. George V. PopescuAssistant Research Professor
FACULTY
email(662) 325-7369
Pace 118
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