Proteomics & Metabolomics
In addition to doing work on existing IGBB projects, the IGBB proteomics staff can
perform a variety of mass spectrometry and other proteomics services for MS State principal investigators and
IGBB collaborators. Such research can be performed through a Proposal Partnership,
a Research Agreement, or the Service Center.
The
IGBB's proteomics staff has considerable expertise in...
- Protein isolation/purification from all types of organisms/tissues
- 1D & 2D gel electrophoresis
- Gel- and non-gel-based mass spectrometry
- Protein identification
- Discovery and characterization of post-translational modifications;
- Quantitative proteomics
- Comparative proteomics & metabolomics
- Western blotting & protein visualization
- Integration of proteomic and nucleic acids data (e.g., proteogenomic
mapping)
- Functional annotation of proteins using Gene Ontology (GO)
standards and procedures
With regard to mass spectrometers, the IGBB's proteomics staff utilizes a ThermoFisher LTQ Orbitrap Velos, a Waters Nano ESI Q-TOF (model Xevo G2-S), and an Applied Biosystems (now ThermoFisher) MALDI TOF TOF. The LTQ Orbitrap Velos and the Nano ESI Q-TOF are fitted with upstream HPLC sample
purification systems.
To discuss the possibility of having the IGBB conduct
proteomics research in collaboration with you, please submit a ticket through the MyIGBB HelpDesk.
An IGBB proteomics consultant will respond to your query as quickly as possible
(usually within 24 hours).
A listing of IGBB Standard Services and their prices -- including information and prices for Training and Self-Service Equipment Usage -- is available in the Standard Services Catalog in MyIGBB and in PDF form via the link below.
ALSO SEE: Genomics (including Transcriptomics) | Biocomputing (Bioinformatics & Computational Biology)
NOTE: PIs are asked to consider whether the participation of an IGBB employee in a project merits that employee's inclusion as a co-author on a resulting manuscript(s). The decision ultimately lies with the PI. However, the IGBB encourages IGBB staff and faculty involved in
Proposal Partnerships and
Research Agreements to discuss/negotiate co-authorship with PIs before starting work on a project.
Michelle LathamBusiness Coordinator
PURCHASING
email(662) 325-4075
Portera

Bacterial diversity analysis of huanglongbing pathogen-infected citrus, using phyloChip arrays and 16S rRNA gene clone library sequencing
IGBB Authors:
Shien Lu, Nian WangPUBLICATION YEAR:
2009IMPACT FACTOR:
4.057CITATION COUNT:
128Sagaram US, Deangelis KM, Trivedi P, Andersen GL, Lu S-E, Wang N (2009) Bacterial diversity analysis of huanglongbing pathogen-infected citrus, using phyloChip arrays and 16S rRNA gene clone library sequencing.
Applied & Environmental Microbiology 75(6): 1566-1574.
DOI:
10.1128/AEM.02404-08EID:
2-s2.0-62149125538PMID: 19151177
DOWNLOAD PDFABSTRACTThe bacterial diversity associated with citrus leaf midribs was characterized for citrus groves that contained the Huanglongbing (HLB) pathogen, which has yet to be cultivated in vitro. We employed a combination of high-density phylogenetic 16S rRNA gene microarrays and 16S rRNA gene clone library sequencing to determine the microbial community composition for symptomatic and asymptomatic citrus midribs. Our results revealed that citrus leaf midribs can support a diversity of microbes. PhyloChip analysis indicated that 47 orders of bacteria in 15 phyla were present in the citrus leaf midribs, while 20 orders in 8 phyla were observed with the cloning and sequencing method. PhyloChip arrays indicated that nine taxa were significantly more abundant in symptomatic midribs than in asymptomatic midribs. "Candidatus Liberibacter asiaticus" was detected at a very low level in asymptomatic plants but was over 200 times more abundant in symptomatic plants. The PhyloChip analysis results were further verified by sequencing 16S rRNA gene clone libraries, which indicated the dominance of "Candidatus Liberibacter asiaticus" in symptomatic leaves. These data implicate "Candidatus Liberibacter asiaticus" as the pathogen responsible for HLB disease.
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The IGBB is an HPC² member center.