Proteomics & Metabolomics
In addition to doing work on existing IGBB projects, the IGBB proteomics staff can perform a variety of mass spectrometry and other proteomics services for MS State principal investigators and IGBB collaborators. Such research can be performed through a Proposal Partnership, a Research Agreement, or the Service Center.
The IGBB's proteomics staff has considerable expertise in...
- Protein isolation/purification from all types of organisms/tissues
- 1D & 2D gel electrophoresis
- Gel- and non-gel-based mass spectrometry
- Protein identification
- Discovery and characterization of post-translational modifications;
- Quantitative proteomics
- Comparative proteomics & metabolomics
- Western blotting & protein visualization
- Integration of proteomic and nucleic acids data (e.g., proteogenomic mapping)
- Functional annotation of proteins using Gene Ontology (GO) standards and procedures
With regard to mass spectrometers, the IGBB's proteomics staff utilizes a ThermoFisher LTQ Orbitrap Velos, a Waters Nano ESI Q-TOF (model Xevo G2-S), and an Applied Biosystems (now ThermoFisher) MALDI TOF TOF. The LTQ Orbitrap Velos and the Nano ESI Q-TOF are fitted with upstream HPLC sample purification systems.
To discuss the possibility of having the IGBB conduct proteomics research in collaboration with you, please submit a ticket through the MyIGBB HelpDesk. An IGBB proteomics consultant will respond to your query as quickly as possible (usually within 24 hours).
A listing of IGBB Standard Services and their prices -- including information and prices for Training and Self-Service Equipment Usage -- is available in the Standard Services Catalog in MyIGBB and in PDF form via the link below.
ALSO SEE: Genomics (including Transcriptomics) | Biocomputing (Bioinformatics & Computational Biology)
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.
