Proteomics & Metabolomics
In addition to doing work on existing IGBB projects, the IGBB proteomics staff can
perform a variety of mass spectrometry and other proteomics services for MS State principal investigators and
IGBB collaborators. Such research can be performed through a Proposal Partnership,
a Research Agreement, or the Service Center.
The
IGBB's proteomics staff has considerable expertise in...
- Protein isolation/purification from all types of organisms/tissues
- 1D & 2D gel electrophoresis
- Gel- and non-gel-based mass spectrometry
- Protein identification
- Discovery and characterization of post-translational modifications;
- Quantitative proteomics
- Comparative proteomics & metabolomics
- Western blotting & protein visualization
- Integration of proteomic and nucleic acids data (e.g., proteogenomic
mapping)
- Functional annotation of proteins using Gene Ontology (GO)
standards and procedures
With regard to mass spectrometers, the IGBB's proteomics staff utilizes a ThermoFisher LTQ Orbitrap Velos, a Waters Nano ESI Q-TOF (model Xevo G2-S), and an Applied Biosystems (now ThermoFisher) MALDI TOF TOF. The LTQ Orbitrap Velos and the Nano ESI Q-TOF are fitted with upstream HPLC sample
purification systems.
To discuss the possibility of having the IGBB conduct
proteomics research in collaboration with you, please submit a ticket through the MyIGBB HelpDesk.
An IGBB proteomics consultant will respond to your query as quickly as possible
(usually within 24 hours).
A listing of IGBB Standard Services and their prices -- including information and prices for Training and Self-Service Equipment Usage -- is available in the Standard Services Catalog in MyIGBB and in PDF form via the link below.
ALSO SEE: Genomics (including Transcriptomics) | Biocomputing (Bioinformatics & Computational Biology)
NOTE: PIs are asked to consider whether the participation of an IGBB employee in a project merits that employee's inclusion as a co-author on a resulting manuscript(s). The decision ultimately lies with the PI. However, the IGBB encourages IGBB staff and faculty involved in
Proposal Partnerships and
Research Agreements to discuss/negotiate co-authorship with PIs before starting work on a project.
Wing patterning gene redefines the mimetic history of Heliconius butterflies
IGBB Authors:
Brian A. CountermanPUBLICATION YEAR:
2011IMPACT FACTOR:
10.425CITATION COUNT:
93Hines HM, Counterman BA, Papa R, Albuquerque de Moura P, Cardoso MZ, Linares M, Mallet J, Reed RD, Jiggins CD, Kronforst MR,McMillan WO (2011) Wing patterning gene redefines the mimetic history of Heliconius butterflies.
Proceedings of the National Academy of Sciences of the United States of America 108(49): 19666-19671.
DOI:
10.1073/pnas.1110096108EID:
2-s2.0-83755207336PMID: 22084094
DOWNLOAD PDFABSTRACTThe mimetic butterflies Heliconius erato and Heliconius melpomene have undergone parallel radiations to form a near-identical patchwork of over 20 different wing-pattern races across the Neotropics. Previous molecular phylogenetic work on these radiations has suggested that similar but geographically disjunct color patterns arose multiple times independently in each species. The neutral markers used in these studies, however, can move freely across color pattern boundaries, and therefore might not represent the history of the adaptive traits as accurately as markers linked to color pattern genes. To assess the evolutionary histories across different loci, we compared relationships among races within H. erato and within H. melpomene using a series of unlinked genes, genes linked to color pattern loci, and optix, a gene recently shown to control red color-pattern variation. We found that although unlinked genes partition populations by geographic region, optix had a different history, structuring lineages by red color patterns and supporting a single origin of red-rayed patterns within each species. Genes closely linked (80-250 kb) to optix exhibited only weak associations with color pattern. This study empirically demonstrates the necessity of examining phenotype-determining genomic regions to understand the history of adaptive change in rapidly radiating lineages. With these refined relationships, we resolve a long-standing debate about the origins of the races within each species, supporting the hypothesis that the red-rayed Amazonian pattern evolved recently and expanded, causing disjunctions of more ancestral patterns.
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.
