The IGBB is a member of the NIH-funded Mississippi IDeA Network of Biomedical Research Excellence (Mississippi INBRE or MS-INBRE), a program designed to build biomedical infrastructure throughout the state. Through MS-INBRE, Mississippi students and faculty at Mississippi's undergraduate institutions are (1) trained in biomedical research techniques, (2) given the opportunity to work with top researchers at Mississippi's major research universities, (3) afforded access to state-of-the-art bioscience equipment, and (4) provided with assistance in preparing grant proposals. The IGBB serves as the MS-INBRE proteomics/computational biology core. For more information about MS-INBRE, click here.
Dr. Zenaida V. MagbanuaSenior Research Associate
GENOMICS
email(662) 325-7647
Pace 120
Differential detergent fractionation for non-electrophoretic eukaryote cell proteomics
IGBB Authors:
Fiona M. McCarthy, Shane C. BurgessPUBLICATION YEAR:
2005IMPACT FACTOR:
7.194CITATION COUNT:
66McCarthy FM, Burgess SC, van den Berg BH, Koter MD, Pharr GT (2005) Differential detergent fractionation for non-electrophoretic eukaryote cell proteomics.
Journal of Proteome Research 4(2): 316-324.
DOI:
10.1021/pr049842dEID:
2-s2.0-17444400484PMID: 15822906
DOWNLOAD PDFABSTRACTDifferential detergent fractionation (DDF), which relies on detergents to sequentially extract proteins from eukaryotic cells, has been used to increase proteome coverage of 2D-PAGE. Here, we used DDF extraction in conjunction with the nonelectrophoretic proteomics method of liquid chromatography and electrospray ionization tandem mass spectrometry. We demonstrate that DDF can be used with 2D-LC ESI MS2 for comprehensive cellular proteomics, including a large proportion of membrane proteins. Compared to some published methods designed to isolate membrane proteins specifically, DDF extraction yields comprehensive proteomes which include twice as many membrane proteins. Two-thirds of these membrane proteins have more than one trans-membrane domain. Since DDF separates proteins based upon their physicochemistry and subcellular localization, this method also provides data useful for functional genome annotation. As more genome sequences are completed, methods which can aid in functional annotation will become increasingly important.
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