Physical Address
IGBB
Portera HPC Building
2 Research Boulevard
Starkville, MS 39759
Mailing Address
IGBB
Portera HPC Building
Box 9627
Mississippi State, MS 39762
Phone/Fax
Phone: 662-325-8278
Fax: 662-325-7692
See Personnel for individual IGBB faculty and staff contact names/numbers/email addresses.
Bacterial diversity analysis of huanglongbing pathogen-infected citrus, using phyloChip arrays and 16S rRNA gene clone library sequencing
IGBB Authors:
Shien Lu, Nian WangPUBLICATION YEAR:
2009IMPACT FACTOR:
4.057CITATION COUNT:
130Sagaram US, Deangelis KM, Trivedi P, Andersen GL, Lu S-E, Wang N (2009) Bacterial diversity analysis of huanglongbing pathogen-infected citrus, using phyloChip arrays and 16S rRNA gene clone library sequencing.
Applied & Environmental Microbiology 75(6): 1566-1574.
DOI:
10.1128/AEM.02404-08EID:
2-s2.0-62149125538PMID: 19151177
DOWNLOAD PDFABSTRACTThe bacterial diversity associated with citrus leaf midribs was characterized for citrus groves that contained the Huanglongbing (HLB) pathogen, which has yet to be cultivated in vitro. We employed a combination of high-density phylogenetic 16S rRNA gene microarrays and 16S rRNA gene clone library sequencing to determine the microbial community composition for symptomatic and asymptomatic citrus midribs. Our results revealed that citrus leaf midribs can support a diversity of microbes. PhyloChip analysis indicated that 47 orders of bacteria in 15 phyla were present in the citrus leaf midribs, while 20 orders in 8 phyla were observed with the cloning and sequencing method. PhyloChip arrays indicated that nine taxa were significantly more abundant in symptomatic midribs than in asymptomatic midribs. "Candidatus Liberibacter asiaticus" was detected at a very low level in asymptomatic plants but was over 200 times more abundant in symptomatic plants. The PhyloChip analysis results were further verified by sequencing 16S rRNA gene clone libraries, which indicated the dominance of "Candidatus Liberibacter asiaticus" in symptomatic leaves. These data implicate "Candidatus Liberibacter asiaticus" as the pathogen responsible for HLB disease.
Dr. Bindu NanduriProfessor
CVM Comparative Biomedical SciencesIGBB Fellow
email(662) 325-4217
Dr. Xueyan ShanAssociate Research Professor
Biochemistry, Molecular Biology, Entomology & Plant PathologyIGBB Affiliate
email(662) 325-2640
The IGBB is supported, in part, by the following units:
The IGBB is an HPC² member center.
